Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
نویسندگان
چکیده
(2)H-(13)C HETCOR MAS NMR is performed on (2)H/(13)C/(15)N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted from (2)H NMR line shapes and T1 in a site-specific manner to elucidate the backbone and side chain molecular dynamics for the MaSp2 GPGXX β-turn regions for spider dragline silk in the dry and wet, supercontracted states.
منابع مشابه
Novel Assembly Properties of Recombinant Spider Dragline Silk Proteins
Spider dragline silk, which exhibits extraordinary strength and toughness, is primarily composed of two related proteins that largely consist of repetitive sequences. In most spiders, the repetitive region of one of these proteins is rich in prolines, which are not present in the repetitive region of the other. The absence of prolines in one component was previously speculated to be essential f...
متن کاملSpider Dragline Silk Molecular Properties and Recombinant Expression
Rising, A. 2007. Spider dragline silk – molecular properties and recombinant production. Doctor’s dissertation. ISSN: 1652-6880, ISBN: 978-91-576-7337-4 Spider dragline silk possesses several desirable features of a biomaterial; it has extraordinary mechanical properties, is biocompatible and biodegradable. It consists of large proteins, major ampullate spidroins (MaSp:s), that contain alternat...
متن کاملA two-dimensional spin-diffusion NMR study on the local structure of a water-soluble model peptide for Nephila clavipes dragline silk (MaSp1) before and after spinning
INTRODUCTION The dragline silk of the golden orb web spider Nephila clavipes has received significant attention because of its remarkable mechanical properties, which include toughness and high tensile strength.1,2 This silk contains two structural proteins designated as major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2).3,4 The dominant MaSp1 can be described as AB block...
متن کاملProtein composition correlates with the mechanical properties of spider ( Argiope trifasciata ) dragline silk.
We investigated the natural variation in silk composition and mechanical performance of the orb-weaving spider Argiope trifasciata at multiple spatial and temporal scales in order to assess how protein composition contributes to the remarkable material properties of spider dragline silk. Major ampullate silk in orb-weaving spiders consists predominantly of two proteins (MaSp1 and MaSp2) with di...
متن کاملHigh-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein
Spider dragline silk is a natural fiber that has excellent tensile properties; however, it is difficult to produce artificially as a long, strong fiber. Here, the spider (Araneus ventricosus) dragline protein gene was cloned and a transgenic silkworm was generated, that expressed the fusion protein of the fibroin heavy chain and spider dragline protein in cocoon silk. The spider silk protein co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 37 شماره
صفحات -
تاریخ انتشار 2014